Comprehensive analysis of transcriptomics and metabolomics to understand tail-suspension-induced myocardial injury in rat.

ABSTRACT:

Background/aims

The effect and underlying mechanism of microgravity on myocardium still poorly understood. The present study aims to reveal the effect and underlying mechanism of tail-suspension-induced microgravity on myocardium of rats.

Methods

Tail-suspension was conducted to simulate microgravity in rats. Echocardiography assay was used to detect cardiac function. The cardiac weight index was measured. Hematoxylin and eosin (HE) staining and transmission electron microscopy assay were conducted to observe the structure of the tissues. RNA sequencing and non-targeted metabolomics was employed to obtain transcriptome and metabolic signatures of heart from tail-suspension-induced microgravity and control rats.

Results

Microgravity induced myocardial atrophy and decre

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9886666 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature