MicroRNAs affecting the susceptibility of melanoma cells to CD8⁺ T cell-mediated cytolysis.

ABSTRACT:

Background

The regulatory functions of microRNAs (miRNAs) in anti-tumour immunity have been mainly described in immune effector cells. Since little is known about miRNA effects on the susceptibility of target cells during T cell-target cell interaction, this study focused on the identification of miRNAs expressed in tumour cells controlling their susceptibility to CD8⁺ T cell-mediated cytotoxicity.

Methods

Luciferase expressing B16F10 melanoma (B16F10 Luci⁺ ) cells transfected with individual miRNAs covering a comprehensive murine miRNA library were screened for their susceptibility to lysis by an established cytotoxic T lymphocyte (CTL) line (5a, clone Nβ) specific for the melanoma-associated antigen tyrosinase-related protein 2. miRNAs with the most p

SUBMITTER: Pane AA 

PROVIDER: S-EPMC9887093 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature