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Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay.

ABSTRACT: Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal's stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported. We aimed to measure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10-60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

SUBMITTER: Aota I

PROVIDER: S-EPMC9887224 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay.

Aota Illia I Yatsuda Chisato C Izawa Ei-Ichi EI

The Journal of veterinary medical science 20221124 1

Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal's stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported. We aimed to measure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was condu ...[more]

PMID: 36418081

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