Antifungal Drug Concentration Impacts the Spectrum of Adaptive Mutations in Candida albicans.

ABSTRACT: Invasive fungal infections are a leading global cause of human mortality. Only three major classes of antifungal drugs are widely used, and resistance to all three classes can arise rapidly. The most widely prescribed antifungal drug, fluconazole, disseminates rapidly and reaches a wide range of concentrations throughout the body. The impact of drug concentration on the spectrum and effect of mutations acquired during adaptation is not known for any fungal pathogen, and how the specific level of a given stress influences the distribution of beneficial mutations has been poorly explored in general. We evolved 144 lineages from three genetically distinct clinical isolates of Candida albicans to four concentrations of fluconazole (0, 1, 8, and 64 μg/ml) and performed comprehensive phenotypic

SUBMITTER: Todd RT 

PROVIDER: S-EPMC9887641 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature