Precision and Accuracy of Receptor Quantification on Synthetic and Biological Surfaces Using DNA-PAINT.

ABSTRACT: Characterization of the number and distribution of biological molecules on 2D surfaces is of foremost importance in biology and biomedicine. Synthetic surfaces bearing recognition motifs are a cornerstone of biosensors, while receptors on the cell surface are critical/vital targets for the treatment of diseases. However, the techniques used to quantify their abundance are qualitative or semi-quantitative and usually lack sensitivity, accuracy, or precision. Detailed herein a simple and versatile workflow based on super-resolution microscopy (DNA-PAINT) was standardized to improve the quantification of the density and distribution of molecules on synthetic substrates and cell membranes. A detailed analysis of accuracy and precision of receptor quantification is presented, based on simulated

SUBMITTER: Riera R 

PROVIDER: S-EPMC9887648 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature