Application of high-density array-based signature-tagged mutagenesis to discover novel Yersinia virulence-associated genes.

ABSTRACT: Yersinia pestis, the causative agent of plague, and the enteropathogen Yersinia pseudotuberculosis have nearly identical nucleotide similarity yet cause markedly different diseases. To investigate this conundrum and to study Yersinia pathogenicity, we developed a high-density oligonucleotide array-based modification of signature-tagged mutagenesis (STM). Y. pseudotuberculosis YPIII mutants constructed with the tagged transposons were evaluated in the murine yersiniosis infection model. The DNA tags were amplified using biotinylated primers and hybridized to high-density oligonucleotide arrays containing DNA complementary to the tags. Comparison of the hybridization signals from input pools and output pools identified a mutant whose relative abundance was significantly reduced in the output

SUBMITTER: Karlyshev AV 

PROVIDER: S-EPMC98877 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature