Cross-species comparison of chemical inhibition of human and Xenopus iodotyrosine deiodinase.

ABSTRACT: The transition to include in vitro-based data in chemical hazard assessment has resulted in the development and implementation of screening assays to cover a diversity of biological pathways, including recently added assays to interrogate chemical disruption of proteins relevant to thyroid signaling pathways. Iodotyrosine deiodinase (IYD), the iodide recycling enzyme, is one such thyroid-relevant endpoint for which a human-based screening assay has recently been developed and used to screen large libraries of chemicals. Presented here is the development of an amphibian IYD inhibition assay and its implementation to conduct a cross-species comparison between chemical inhibition of mammalian and non-mammalian IYD enzyme activity. The successful development of an amphibian IYD inhibition assa

SUBMITTER: Olker JH 

PROVIDER: S-EPMC9887787 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature