LncRNA AK089514/miR-125b-5p/TRAF6 axis mediates macrophage polarization in allergic asthma.

ABSTRACT:

Background

Micro RNA (miRNA) plays important roles in macrophage polarization. However, the manner in which miRNA regulate macrophage polarization in response to dermatophagoides farinae protein 1(Der f1)-induced asthma has not been defined. This study aims to explore the role of miRNAs in regulating macrophages in asthma.

Methods

The microRNAs which may regulate asthma were selectd by Microarrays. The function of miR-125b-5p in macrophage and Der f1-induced asthma were detected in vivo experiment. The long non coding RNA (lncRNA) AK089514/miR-125b-5p/TRAF6 axis was predicted by bioinformatics and confirmed by dual luciferase reporter assay.

Results

In this study, we found that miR-125b-5p is highly expressed in M2 macrophages and bronchoalveolar lavage fluid (BALF) c

SUBMITTER: Zhu X 

PROVIDER: S-EPMC9887860 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature