E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis.

ABSTRACT:

Introduction

Super-enhancer-associated lncRNAs play important roles in the occurrence and development of malignant tumors, including hepatocellular carcinoma (HCC).

Objectives

The current work aimed to identify and characterize super-enhancer-associated lncRNAs in the pathogenesis of HCC.

Methods

H3K27ac ChIP-seq data from HepG2 cell line and two HCC tissues were used to identify super-enhancer-associated lncRNAs in HCC. JQ-1 treatment and CRISPR-dCas9 system were performed to confirm super-enhancer activity. Quantitative real-time PCR (qPCR), ChIP-qPCR, and dual-luciferase reporter system assay demonstrated the regulation of E2F1 on super-enhancer. Functional loss experiment was used to identify the function of LINC01004.

Results

In this study, we identified a

SUBMITTER: Li J 

PROVIDER: S-EPMC9887888 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature