The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome.

ABSTRACT: Metagenomics offers the possibility to screen for versatile biocatalysts. In this study, the microbial community of the Sorghum bicolor rhizosphere was spiked with technical cashew nut shell liquid, and after incubation, the environmental DNA (eDNA) was extracted and subsequently used to build a metagenomic library. We report the biochemical features and crystal structure of a novel esterase from the family IV, EH₀, retrieved from an uncultured sphingomonad after a functional screen in tributyrin agar plates. EH₀ (optimum temperature [T_opt], 50°C; melting temperature [T_m], 55.7°C; optimum pH [pH_opt], 9.5) was stable in the presence of 10 to 20% (vol/vol) organic solvents and exhibited hydrolytic activity against p-

SUBMITTER: Distaso M 

PROVIDER: S-EPMC9888213 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature