Clone Dynamics and Its Application for the Diagnosis of Enzootic Bovine Leukosis.

ABSTRACT: Bovine leukemia virus (BLV) infection results in polyclonal expansion of infected B lymphocytes, and ~5% of infected cattle develop enzootic bovine leukosis (EBL). Since BLV is a retrovirus, each individual clone can be identified by using viral integration sites. To investigate the distribution of tumor cells in EBL cattle, we performed viral integration site analysis by using a viral DNA capture-sequencing method. We found that the same tumor clones existed in peripheral blood, with a dominance similar to that in lymphoma tissue. Additionally, we observed that multiple tumor tissues from different sites harbored the identical clones, indicating that tumor cells can circulate and distribute systematically in EBL cattle. To investigate clonal expansion of BLV-infected cells during a long l

SUBMITTER: Hossain MB 

PROVIDER: S-EPMC9888225 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature