N-Glycosylation of Rotavirus NSP4 Protein Affects Viral Replication and Pathogenesis.

ABSTRACT: Rotavirus (RV), the most common cause of gastroenteritis in children, carries a high economic and health burden worldwide. RV encodes six structural proteins and six nonstructural proteins (NSPs) that play different roles in viral replication. NSP4, a multifunctional protein involved in various viral replication processes, has two conserved N-glycosylation sites; however, the role of glycans remains elusive. Here, we used recombinant viruses generated by a reverse genetics system to determine the role of NSP4 N-glycosylation during viral replication and pathogenesis. The growth rate of recombinant viruses that lost one glycosylation site was as high as that of the wild-type virus. However, a recombinant virus that lost both glycosylation sites (glycosylation-defective virus) showed attenua

SUBMITTER: Nurdin JA 

PROVIDER: S-EPMC9888287 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature