Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease.

ABSTRACT:

Objectives

Subfornical organ (SFO) is vital in chronic kidney disease (CKD) progression caused by high salt levels. The current study investigated the effects of high salt on phosphoproteomic changes in SFO in CKD rats.

Methods

5/6 nephrectomized rats were fed a normal-salt diet (0.4%) (NC group) or a high-salt diet (4%) (HC group) for three weeks, while sham-operated rats were fed a normal-salt diet (0.4%) (NS group). For phosphoproteomic analysis of SFO in different groups, TiO₂ enrichment, isobaric tags for relative and absolute quantification (iTRAQ) labeling, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used.

Results

There were 6808 distinct phosphopeptides found, which corresponded to 2661 phosphoproteins. NC group had 168 upregu

SUBMITTER: Wang X 

PROVIDER: S-EPMC9888458 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature