Role of circular RNA cdr1as in modulation of macrophage phenotype.

ABSTRACT:

Aims

Macrophages are crucial for the initiation and resolution of an inflammatory response. Non-coding circular RNAs are ubiquitously expressed in mammalian tissue, highly conserved among species, and recently implicated in the regulation of macrophage activation. We sought to determine whether circRNAs modulate monocyte/macrophage biology and function.

Materials and methods

We performed circRNA microarray analyses to assess transcriptome changes using RNA isolated from bone marrow derived macrophages polarized to a pro-inflammatory phenotype (INFγ + TNFα) or an anti-inflammatory phenotype (IL-10, IL-4, and TGF-β). Among differentially expressed circRNAs, circ-Cdr1as was chosen for further investigation. Additionally, we performed loss or gain of function studies to investig

SUBMITTER: Gonzalez C 

PROVIDER: S-EPMC9888537 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature