Identification of the Hub Genes Involved in Stem Cell Treatment for Intervertebral Disc Degeneration: A Conjoint Analysis of Single-Cell and Machine Learning.

ABSTRACT: Intervertebral disc degeneration (IDD), which is distinguished by a variety of pathologic alterations, is the major cause of low back pain (LBP). Nonetheless, preventative measures or therapies that may delay IDD are scarcely available. In this study, we sought to identify new diagnostic biological markers for IDD. In this first-of-a-kind study combining machine learning, stem cell treatment samples and single-cell sequencing data were collected. Differentially expressed genes (DEGs) were detected from the treatment group and clusters. To filter potential markers, support vector machine analysis and LASSO were performed. LAPTM5 was found to be the hub gene for IDD. In addition, the results of single-cell sequencing demonstrated the critical function of stem cells in IDD. Finally, we found

SUBMITTER: Chen J 

PROVIDER: S-EPMC9889147 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature