Incorporating microglia-like cells in human induced pluripotent stem cell-derived retinal organoids.

ABSTRACT: Microglia are the primary resident immune cells in the retina. They regulate neuronal survival and synaptic pruning making them essential for normal development. Following injury, they mediate adaptive responses and under pathological conditions they can trigger neurodegeneration exacerbating the effect of a disease. Retinal organoids derived from human induced pluripotent stem cells (hiPSCs) are increasingly being used for a range of applications, including disease modelling, development of new therapies and in the study of retinogenesis. Despite many similarities to the retinas developed in vivo, they lack some key physiological features, including immune cells. We engineered an hiPSC co-culture system containing retinal organoids and microglia-like (iMG) cells and tested their retinal i

SUBMITTER: Chichagova V 

PROVIDER: S-EPMC9889627 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature