Recovery of Salmonella bacterial isolates from pooled fecal samples from horses.

ABSTRACT:

Background

It is important to determine if a horse is shedding Salmonella spp., but a complete culture series can be cost prohibitive.

Objectives

Determine the optimal pooling technique to maintain high sensitivity of Salmonella spp. culture using spiked samples, and then demonstrate the efficacy of this protocol on clinical submissions.

Hypothesis

Pooled fecal samples are as sensitive as 5 individual cultures for the detection of Salmonella shedding.

Animals

A single Salmonella-negative horse from the university herd, and 19 hospitalized horses.

Methods

Salmonella-free fecal samples were spiked with different amounts of Salmonella spp. (10² , 10³ , 10⁴ , and 10⁵ colony forming units [cfu]) and homogenized to e

SUBMITTER: Goni JI 

PROVIDER: S-EPMC9889685 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature