Robust and adjustable dynamic scattering compensation for high-precision deep tissue optogenetics.

ABSTRACT: The development of high-precision optogenetics in deep tissue is limited due to the strong optical scattering induced by biological tissue. Although various wavefront shaping techniques have been developed to compensate the scattering, it is still a challenge to non-invasively characterize the dynamic scattered optical wavefront inside the living tissue. Here, we present a non-invasive scattering compensation system with fast multidither coherent optical adaptive technique (fCOAT), which allows the rapid wavefront correction and stable focusing in dynamic scattering medium. We achieve subcellular-resolution focusing through 500-μm-thickness brain slices, or even three pieces overlapped mouse skulls after just one iteration with a 589 nm CW laser. Further, focusing through dynamic scatterin

SUBMITTER: Li Z 

PROVIDER: S-EPMC9889738 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature