Using click chemistry to study microbial ecology and evolution.

ABSTRACT: Technological advances have largely driven the revolution in our understanding of the structure and function of microbial communities. Culturing, long the primary tool to probe microbial life, was supplanted by sequencing and other -omics approaches, which allowed detailed quantitative insights into species composition, metabolic potential, transcriptional activity, secretory responses and more. Although the ability to characterize "who's there" has never been easier or cheaper, it remains technically challenging and expensive to understand what the diverse species and strains that comprise microbial communities are doing in situ, and how these behaviors change through time. Our aim in this brief review is to introduce a developing toolkit based on click chemistry that can accelerate and r

SUBMITTER: van Kasteren S 

PROVIDER: S-EPMC9889756 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature