Modelling metastatic colonization of cholangiocarcinoma organoids in decellularized lung and lymph nodes.

ABSTRACT: Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. The metastasis of CCA cancer cells to distant organs, commonly lung and lymph nodes, drastically reduces overall survival. However, mechanistic insight how CCA invades these metastatic sites is still lacking. This is partly because currently available models fail to mimic the complexity of tissue-specific environments for metastatic CCA. To create an in vitro model in which interactions between epithelial tumor cells and their surrounding extracellular matrix (ECM) can be studied in a metastatic setting, we combined patient-derived CCA organoids (CCAOs) (n=3) with decellularized human lung (n=3) and decellularized human lymph node (n=13). Decellularization resulted in remo

SUBMITTER: van Tienderen GS 

PROVIDER: S-EPMC9890173 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature