Knockdown of ATF3 suppresses the progression of ischemic stroke through inhibiting ferroptosis.

ABSTRACT:

Objective

Current therapies towards ischemic stroke (IS) are still not satisfied, and alternative strategies targeting ferroptosis may be another choice. The purpose of this study is to screen potential ferroptosis-related genes involving in IS.

Methods

A rat model of IS was established via middle cerebral artery occlusion. Differentially expressed genes (DEGs) were screened from the model rats through transcriptional sequencing. Among the isolated DEGs, the expression of several attractive DEGs relating with ischemic injury was confirmed by qRT-PCR. Then, ATF3 relating with both IS and ferroptosis was selected a candidate gene for functional assays. After knockdown of ATF3 in the model rats, the infarction, histopathology, apoptosis, and ferroptosis in brain tissues

SUBMITTER: Ye J 

PROVIDER: S-EPMC9890179 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature