Assessing Cellular and Transcriptional Diversity of Ileal Mucosa Among Treatment-Naive and Treated Crohn's Disease.

ABSTRACT:

Background

Crohn's disease is a lifelong disease characterized by chronic inflammation of the gastrointestinal tract. Defining the cellular and transcriptional composition of the mucosa at different stages of disease progression is needed for personalized therapy in Crohn's.

Methods

Ileal biopsies were obtained from (1) control subjects (n = 6), (2) treatment-naïve patients (n = 7), and (3) established (n = 14) Crohn's patients along with remission (n = 3) and refractory (n = 11) treatment groups. The biopsies processed using 10x Genomics single cell 5' yielded 139 906 cells. Gene expression count matrices of all samples were analyzed by reciprocal principal component integration, followed by clustering analysis. Manual annotations of the clusters were performed using canoni

SUBMITTER: Maddipatla SC 

PROVIDER: S-EPMC9890215 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature