Characterization of Kinetic Isotope Effects and Label Loss in Deuterium-Based Isotopic Labeling Studies.

ABSTRACT: Deuterium metabolic imaging (DMI) is a novel, 3D, magnetic resonance (MR)-based method to map metabolism of deuterated substrates in vivo. The replacement of protons with deuterons could potentially lead to kinetic isotope effects (KIEs) in which metabolic rates of deuterated substrates are reduced due to the presence of a heavier isotope. Knowledge of the extent of KIE in vivo and ²H label loss due to exchange reactions is required for DMI-based measurements of absolute metabolic rates. Here the deuterium KIE and label loss in vivo are investigated for glucose and acetate using a double substrate/double labeling strategy and ¹H-decoupled ¹³C NMR in rat glioma cells and rat brain tissue metabolite extracts. The unique spectral patterns

SUBMITTER: de Graaf RA 

PROVIDER: S-EPMC9890388 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature