Septin 9 controls CCNB1 stabilization via APC/C^CDC20 during meiotic metaphase I/anaphase I transition in mouse oocytes.

ABSTRACT: The anaphase promoting complex/cyclosome (APC/C) and its cofactors CDH1 and CDC20 regulate the accumulation/degradation of CCNB1 during mouse oocyte meiotic maturation. Generally, the CCNB1 degradation mediated by APC/C^CDC20 activity is essential for the transition from metaphase to anaphase. Here, by using siRNA and mRNA microinjection, as well as time-lapse live imaging, we showed that Septin 9, which mediates the binding of septins to microtubules, is critical for oocyte meiotic cell cycle progression. The oocytes were arrested at the MI stage and the connection between chromosome kinetochores and spindle microtubules was disrupted after Septin 9 depletion. As it is well known that spindle assembly checkpoint (SAC) is an important regulator of the MI-AI transition, we thus de

SUBMITTER: Chen L 

PROVIDER: S-EPMC9890537 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature