An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution.

ABSTRACT:

Background

Live imaging is the gold standard for determining how cells give rise to organs. However, tracking many cells across whole organs over large developmental time windows is extremely challenging. In this work, we provide a comparably simple method for confocal live imaging entire Arabidopsis thaliana first leaves across early development. Our imaging method works for both wild-type leaves and the complex curved leaves of the jaw-1D mutant.

Results

We find that dissecting the cotyledons, affixing a coverslip above the samples and mounting samples with perfluorodecalin yields optimal imaging series for robust cellular and organ level analysis. We provide details of our complementary image processing steps in MorphoGraphX software for segmenting, tracking lineages, and

SUBMITTER: Harline K 

PROVIDER: S-EPMC9890716 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature