Construction of multiphasic membraneless organelles towards spontaneous spatial segregation and directional flow of biochemical reactions.

ABSTRACT: Many intracellular membraneless organelles (MLOs) appear to adapt a hierarchical multicompartment organization for efficient coordination of highly complex reaction networks. Recapitulating such an internal architecture in biomimetic platforms is, therefore, an important step to facilitate the functional understanding of MLOs and to enable the design of advanced microreactors. Herein, we present a modular bottom-up approach for building synthetic multiphasic condensates using a set of engineered multivalent polymer-oligopeptide hybrids. These hybrid constructs exhibit dynamic phase separation behaviour generating membraneless droplets with a subdivided interior featuring distinct chemical and physical properties, whereby a range of functional biomolecules can be spontaneously enriched and

SUBMITTER: Zhorabek F 

PROVIDER: S-EPMC9890938 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature