Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts.

ABSTRACT: Photosynthetic organelles offer attractive features for engineering small molecule bioproduction by their ability to convert solar energy into chemical energy required for metabolism. The possibility to couple biochemical production directly to photosynthetic assimilation as a source of energy and substrates has intrigued metabolic engineers. Specifically, the chemical diversity found in plants often relies on cytochrome P450-mediated hydroxylations that depend on reductant supply for catalysis and which often lead to metabolic bottlenecks for heterologous production of complex molecules. By directing P450 enzymes to plant chloroplasts one can elegantly deal with such redox prerequisites. In this study, we explore the capacity of the plant photosynthetic machinery to drive P450-dependent f

SUBMITTER: Mellor SB 

PROVIDER: S-EPMC9890960 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature