Hammerhead ribozyme-based U-insertion and deletion RNA editing assays for multiplexing in HTS applications.

ABSTRACT: Untranslatable mitochondrial transcripts in kinetoplastids are decrypted post-transcriptionally through an RNA editing process that entails uridine insertion/deletion. This unique stepwise process is mediated by the editosome, a multiprotein complex that is a validated drug target of considerable interest in addressing the unmet medical needs for kinetoplastid diseases. With that objective, several in vitro RNA editing assays have been developed, albeit with limited success in discovering potent inhibitors. This manuscript describes the development of three hammerhead ribozyme (HHR) FRET reporter-based RNA editing assays for precleaved deletion, insertion, and ligation assays that bypass the rate-limiting endonucleolytic cleavage step, providing information on U-deletion, U-insertion, and

SUBMITTER: Rostamighadi M 

PROVIDER: S-EPMC9891259 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature