A selective and sensitive detection system for 4-thiouridine modification in RNA.

ABSTRACT: 4-Thiouridine (s⁴U) is a modified nucleoside, found at positions 8 and 9 in tRNA from eubacteria and archaea. Studies of the biosynthetic pathway and physiological role of s⁴U in tRNA are ongoing in the tRNA modification field. s⁴U has also recently been utilized as a biotechnological tool for analysis of RNAs. Therefore, a selective and sensitive system for the detection of s⁴U is essential for progress in the fields of RNA technologies and tRNA modification. Here, we report the use of biotin-coupled 2-aminoethyl-methanethiosulfonate (MTSEA biotin-XX) for labeling of s⁴U and demonstrate that the system is sensitive and quantitative. This technique can be used without denaturation; however, addition of a denaturation step improves the

SUBMITTER: Sugio Y 

PROVIDER: S-EPMC9891261 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature