Identification and characterization of plasmids carrying the mobile colistin resistance gene mcr-1 using optical DNA mapping.

ABSTRACT:

Objectives

Colistin is a last-resort antibiotic, but there has been a rapid increase in colistin resistance, threatening its use in the treatment of infections with carbapenem-resistant Enterobacterales (CRE). Plasmid-mediated colistin resistance, in particular the mcr-1 gene, has been identified and WGS is the go-to method in identifying plasmids carrying mcr-1 genes. The goal of this study is to demonstrate the use of optical DNA mapping (ODM), a fast, efficient and amplification-free technique, to characterize plasmids carrying mcr-1.

Methods

ODM is a single-molecule technique, which we have demonstrated can be used for identifying plasmids harbouring antibiotic resistance genes. We here applied the technique to plasmids isolated from 12 clinic

SUBMITTER: Kk S 

PROVIDER: S-EPMC9891347 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature