Dataset Information

Upregulated expression of FFAR2 and SOC3 genes is associated with gout.

ABSTRACT:

Objective

To examine the expression of Free fatty acid receptor 2 (FFAR2) and Suppressor of cytokine signalling 3 (SOCS3) genes in asymptomatic hyperuricaemia (AH), AH with MSU crystal deposition, inter-critical gout and gout flare.

Methods

Study participants (n = 120) comprised 34 people with serum urate (SU) <360 μmol/l, 69 with AH ± MSU crystal deposition and 17 with a gout flare. Sixteen of the 17 patients with a gout flare attended a second visit 6-12 weeks later. Gene expression levels were assessed using RT-qPCR and results computed as fold changes (FC) after normalization to the reference gene.

Results

FFAR2 was significantly upregulated during gout flares (FC = 2.9) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, P < 0.0001 for each comparison). FFAR2 was also significantly upregulated during inter-critical gout (FC = 1.8) compared with normal SU, AH and AH + MSU (FC = 1.1, P < 0.001 for each comparison). SOCS3 was significantly upregulated during gout flares (FC = 3.4) compared with normal SU, AH, and AH + MSU crystal deposition (FC = 1.1, 1.1 and 1.2, respectively, P < 0.0001 for each comparison). SOCS3 was also upregulated during inter-critical gout (FC = 2.1) compared with normal SU (P = 0.02) and AH (P = 0.006) (FC = 1.1 and 1.2, respectively). FFAR2 expression was upregulated during gout flare compared with inter-critical gout and SOCS3 expression showed negative correlation with flare duration (r = -0.49, P < 0.05).

Conclusion

FFAR2 upregulation is associated with gout and may trigger gout flares. SOCS3 may have a role in amelioration of gout flares.

SUBMITTER: Orji OC

PROVIDER: S-EPMC9891400 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature

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Publications

Upregulated expression of FFAR2 and SOC3 genes is associated with gout.

Orji Oliver C OC López-Domínguez Maria B MB Sandoval-Plata Gabriela G Guetta-Baranes Tamar T Valdes Ana M AM Doherty Michael M Morgan Kevin K Abhishek Abhishek A

Rheumatology (Oxford, England) 20230201 2

<h4>Objective</h4>To examine the expression of Free fatty acid receptor 2 (FFAR2) and Suppressor of cytokine signalling 3 (SOCS3) genes in asymptomatic hyperuricaemia (AH), AH with MSU crystal deposition, inter-critical gout and gout flare.<h4>Methods</h4>Study participants (n = 120) comprised 34 people with serum urate (SU) <360 μmol/l, 69 with AH ± MSU crystal deposition and 17 with a gout flare. Sixteen of the 17 patients with a gout flare attended a second visit 6-12 weeks later. Gene expres ...[more]

PMID: 35731142

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Dataset Information

Upregulated expression of FFAR2 and SOC3 genes is associated with gout.

Objective

Methods

Results

Conclusion

Publications

Upregulated expression of FFAR2 and SOC3 genes is associated with gout.

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