Identification and functional characterization of imbalanced osteoarthritis-associated fibronectin splice variants.

ABSTRACT:

Objective

To identify FN1 transcripts associated with OA pathophysiology and investigate the downstream effects of modulating FN1 expression and relative transcript ratio.

Methods

FN1 transcriptomic data was obtained from our previously assessed RNA-seq dataset of lesioned and preserved OA cartilage samples from the Research osteoArthritis Articular Cartilage (RAAK) study. Differential transcript expression analysis was performed on all 27 FN1 transcripts annotated in the Ensembl database. Human primary chondrocytes were transduced with lentiviral particles containing short hairpin RNA (shRNA) targeting full-length FN1 transcripts or non-targeting shRNA. Subsequently, matrix deposition was induced in our 3D in vitro neo-cartilage model. Effects of changes in the FN1 transcri

SUBMITTER: van Hoolwerff M 

PROVIDER: S-EPMC9891405 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature