Mitochondria transplant therapy improves regeneration and restoration of injured skeletal muscle.

ABSTRACT:

Background

Injection of exogenous mitochondria has been shown to improve the ischaemia-damaged myocardium, but the effect of mitochondrial transplant therapy (MTT) to restore skeletal muscle mass and function has not been tested following neuromuscular injury. Therefore, we tested the hypothesis that MTT would improve the restoration of muscle function after injury.

Methods

BaCl₂ was injected into the gastrocnemius muscle of one limb of 8-12-week-old C57BL/6 mice to induce damage without injury to the resident stem cells. The contralateral gastrocnemius muscle was injected with phosphate-buffered saline (PBS) and served as the non-injured intra-animal control. Mitochondria were isolated from donor mice. Donor mitochondria were suspended in PBS or PBS without mitoc

SUBMITTER: Alway SE 

PROVIDER: S-EPMC9891964 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature