Profound phenotypic and epigenetic heterogeneity of the HIV-1-infected CD4⁺ T cell reservoir.

ABSTRACT: Understanding the complexity of the long-lived HIV reservoir during antiretroviral therapy (ART) remains a considerable impediment in research towards a cure for HIV. To address this, we developed a single-cell strategy to precisely define the unperturbed peripheral blood HIV-infected memory CD4⁺ T cell reservoir from ART-treated people living with HIV (ART-PLWH) via the presence of integrated accessible proviral DNA in concert with epigenetic and cell surface protein profiling. We identified profound reservoir heterogeneity within and between ART-PLWH, characterized by new and known surface markers within total and individual memory CD4⁺ T cell subsets. We further uncovered new epigenetic profiles and transcription factor motifs enriched in HIV-infected cells that su

SUBMITTER: Wu VH 

PROVIDER: S-EPMC9892009 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature