Mitochondrial complexome reveals quality-control pathways of protein import.

ABSTRACT: Mitochondria have crucial roles in cellular energetics, metabolism, signalling and quality control^1-4. They contain around 1,000 different proteins that often assemble into complexes and supercomplexes such as respiratory complexes and preprotein translocases^1,3-7. The composition of the mitochondrial proteome has been characterized^1,3,5,6; however, the organization of mitochondrial proteins into stable and dynamic assemblies is poorly understood for major parts of the proteome^1,4,7. Here we report quantitative mapping of mitochondrial protein assemblies using high-resolution complexome profiling of more than 90% of the yeast mitochondrial proteome, termed MitCOM. An analysis of the MitCOM dataset resolves >5,200 protein peaks with an average of

SUBMITTER: Schulte U 

PROVIDER: S-EPMC9892010 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature