CCR2-positive monocytes contribute to the pathogenesis of early diabetic retinopathy in mice.

ABSTRACT:

Aims/hypothesis

Accumulating evidence suggests that leucocytes play a critical role in diabetes-induced vascular lesions and other abnormalities that characterise the early stages of diabetic retinopathy. However, the role of monocytes has yet to be fully investigated; therefore, we used Ccr2^-/- mice to study the role of CCR2⁺ inflammatory monocytes in the pathogenesis of diabetes-induced degeneration of retinal capillaries.

Methods

Experimental diabetes was induced in wild-type and Ccr2^-/- mice using streptozotocin. After 2 months, superoxide levels, expression of inflammatory genes, leucostasis, leucocyte- and monocyte-mediated cytotoxicity against retinal endothelial cell death, retinal thickness and visual function were evaluated. Retinal

SUBMITTER: Saadane A 

PROVIDER: S-EPMC9892100 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature