Intra-articular kinetics of a cartilage targeting cationic PEGylated protein for applications in drug delivery.

ABSTRACT:

Objectives

Cartilage targeting cationic glycoprotein Avidin was PEGylated to synthesize a multi-arm Avidin (mAv) nano-construct with high drug loading content. Here we investigate mAv biodistribution and kinetics over a 7-day period following intra-articular (IA) administration in rat knee joints.

Methods

Labeled mAv was injected into healthy rat knees, and joint tissues (articular cartilage, menisci, ligaments, tendons, fat pad) were harvested following sacrifice at 6 h, 1, 4 and 7 days. Its IA biodistribution and retention were measured using fluorescence microscopy. Tissue localization was compared in young vs old rats by immunohistochemistry. mAv chondrotoxicity and immune response were evaluated to determine safe carrier dose limits.

Results

mAv penetrated throug

SUBMITTER: He T 

PROVIDER: S-EPMC9892226 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature