G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation.

ABSTRACT:

Background & aims

Although T-cell intrinsic expression of G9a has been associated with murine intestinal inflammation, mechanistic insight into the role of this methyltransferase in human T-cell differentiation is ill defined, and manipulation of G9a function for therapeutic use against inflammatory disorders is unexplored.

Methods

Human naive T cells were isolated from peripheral blood and differentiated in vitro in the presence of a G9a inhibitor (UNC0642) before being characterized via the transcriptome (RNA sequencing), chromatin accessibility (assay for transposase-accessible chromatin by sequencing), protein expression (cytometry by time of flight, flow cytometry), metabolism (mitochondrial stress test, ultrahigh performance liquid chromatography-tandem mas spectroscop

SUBMITTER: Ramos GP 

PROVIDER: S-EPMC9892272 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature