Proteomic profiling of sweat in patients with cystic fibrosis provides new insights into epidermal homoeostasis.

ABSTRACT:

Background

A high proportion of patients with Cystic Fibrosis (CF) also present the rare skin disease aquagenic palmoplantar keratoderma. A possible link between this condition and absence of a functional CF Transmembrane conductance Regulator protein in the sweat acinus and collecting duct remains unknown.

Methods

In-depth characterization of sweat proteome profiles was performed in 25 CF patients compared to 12 healthy controls. A 20 μL sweat sample was collected after pilocarpine iontophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomic analysis was performed.

Results

Sweat proteome profile of CF patients was significantly different from that of healthy subjects with 57 differentially expressed proteins. Cystic Fibrosis sweat proteome wa

SUBMITTER: Cornet M 

PROVIDER: S-EPMC9892418 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature