Differentiation of subnucleus-sized oligomers and nucleation-competent assemblies of the Aβ peptide.

ABSTRACT: A significant feature of Alzheimer's disease is the formation of amyloid deposits in the brain consisting mainly of misfolded derivatives of proteolytic cleavage products of the amyloid precursor protein amyloid-β (Aβ) peptide. While high-resolution structures already exist for both the monomer and the amyloid fibril of the Aβ peptide, the mechanism of amyloid formation itself still defies precise characterization. In this study, low and high molecular weight oligomers (LMWOs and HMWOs) were identified by sedimentation velocity analysis, and for the first time, the temporal evolution of oligomer size distributions was correlated with the kinetics of amyloid formation as determined by thioflavin T-binding studies. LMWOs of subnucleus size contain fewer than seven monomer units and exist alo

SUBMITTER: Pauly T 

PROVIDER: S-EPMC9892607 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature