Circulating levels and the bioactivity of miR-30b increase during pubertal progression in boys.

ABSTRACT:

Background

Puberty marks the transition from childhood to adulthood and is initiated by activation of a pulsatile GnRH secretion from the hypothalamus. MKRN3 functions as a pre-pubertal break on the GnRH pulse generator and hypothalamic expression and circulating levels of MKRN3 decrease peri-pubertally. In rodents, microRNA miR-30b seems to directly target hypothalamic MKRN3 expression - and in boys, circulating levels of miR-30b-5p increase when puberty is pharmacologically induced. Similarly, miR-200b-3p and miR-155-5p have been suggested to inhibit expression of other proteins potentially involved in the regulation of GnRH secretion. Here we measure circulating levels of these three miRNAs as boys progress through puberty.

Materials and methods

Forty-six boys from

SUBMITTER: Morup N 

PROVIDER: S-EPMC9893272 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature