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NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization.

ABSTRACT: The molecular weight, purity, and functionalization of polyethylene glycols are often characterized by ¹H NMR spectroscopy. Oft-forgotten, the typical ¹H NMR pulse sequence is not ¹³C decoupled. Hence, for large polymers, the ¹³C coupled ¹H peaks arising from the repeating units have integrations comparable to that of the ¹H of the terminal groups. Ignoring this coupling leads to erroneous assignments. Once correctly assigned, these ¹³C coupled ¹H peaks can be used to determine both the molecular weight of the polymer and the efficacy of conjugation of a terminal moiety more accurately than the uncoupled ¹H of the repeating unit.

SUBMITTER: Pasek-Allen JL

PROVIDER: S-EPMC9893458 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization.

Pasek-Allen Jacqueline L JL Wilharm Randall K RK Bischof John C JC Pierre Valérie C VC

ACS omega 20230118 4

The molecular weight, purity, and functionalization of polyethylene glycols are often characterized by 1H NMR spectroscopy. Oft-forgotten, the typical 1H NMR pulse sequence is not 13C decoupled. Hence, for large polymers, the 13C coupled 1H peaks arising from the repeating units have integrations comparable to that of the 1H of the terminal groups. Ignoring this coupling leads to erroneous assignments. Once correctly assigned, the ...[more]

PMID: 36743059

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Project description:In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV-Vis spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.

Dataset Information

NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization.

Publications

NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization.

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