Integrative single-cell RNA-seq and ATAC-seq analysis of myogenic differentiation in pig.

ABSTRACT:

Background

Skeletal muscle development is a multistep process whose understanding is central in a broad range of fields and applications, from the potential medical value to human society, to its economic value associated with improvement of agricultural animals. Skeletal muscle initiates in the somites, with muscle precursor cells generated in the dermomyotome and dermomyotome-derived myotome before muscle differentiation ensues, a developmentally regulated process that is well characterized in model organisms. However, the regulation of skeletal muscle ontogeny during embryonic development remains poorly defined in farm animals, for instance in pig. Here, we profiled gene expression and chromatin accessibility in developing pig somites and myotomes at single-cell resolution.

R

SUBMITTER: Cai S 

PROVIDER: S-EPMC9893630 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature