The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression.

ABSTRACT:

Background

Brain microvascular endothelial cells (BMECs) play a major role in the blood-brain barrier (BBB), and are critical for establishing an in vitro BBB model. Currently, iPSC-derived BMECs (iBMECs) have been used to construct in vitro BBB models with physiological barrier functions, such as high trans-endothelial electrical resistance (TEER) and expression of transporter proteins. However, the relatively low p-glycoprotein (P-gp) level and a decrease in the efflux ratio of its substrates in iBMECs suggest their immature nature. Therefore, more mature iBMECs by optimizing the differentiation induction protocol is beneficial for establishing a more reliable in vitro BBB model for studying central nervous system (CNS) drug transport.

Methods

To identify human brain endot

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9893670 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature