A genetic tool to express long fungal biosynthetic genes.

ABSTRACT:

Background

Secondary metabolites (SMs) from mushroom-forming fungi (Basidiomycota) and early diverging fungi (EDF) such as Mucoromycota are scarcely investigated. In many cases, production of SMs is induced by unknown stress factors or is accompanied by seasonable developmental changes on fungal morphology. Moreover, many of these fungi are considered as non-culturable under laboratory conditions which impedes investigation into SM. In the post-genomic era, numerous novel SM genes have been identified especially from EDF. As most of them encode multi-module enzymes, these genes are usually long which limits cloning and heterologous expression in traditional hosts.

Results

An expression system in Aspergillus niger is presented that is suitable for the production of SMs from b

SUBMITTER: Kirchgaessner L 

PROVIDER: S-EPMC9893682 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature