The Vacc-SeqQC project: Benchmarking RNA-Seq for clinical vaccine studies.

ABSTRACT:

Introduction

Over the last decade, the field of systems vaccinology has emerged, in which high throughput transcriptomics and other omics assays are used to probe changes of the innate and adaptive immune system in response to vaccination. The goal of this study was to benchmark key technical and analytical parameters of RNA sequencing (RNA-seq) in the context of a multi-site, double-blind randomized vaccine clinical trial.

Methods

We collected longitudinal peripheral blood mononuclear cell (PBMC) samples from 10 subjects before and after vaccination with a live attenuated Francisella tularensis vaccine and performed RNA-Seq at two different sites using aliquots from the same sample to generate two replicate datasets (5 time points for 50 samples each). We evaluated t

SUBMITTER: Goll JB 

PROVIDER: S-EPMC9893923 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature