Computational design of custom therapeutic cells to correct failing human cardiomyocytes.

ABSTRACT:

Background

Myocardial delivery of non-excitable cells-namely human mesenchymal stem cells (hMSCs) and c-kit⁺ cardiac interstitial cells (hCICs)-remains a promising approach for treating the failing heart. Recent empirical studies attempt to improve such therapies by genetically engineering cells to express specific ion channels, or by creating hybrid cells with combined channel expression. This study uses a computational modeling approach to test the hypothesis that custom hypothetical cells can be rationally designed to restore a healthy phenotype when coupled to human heart failure (HF) cardiomyocytes.

Methods

Candidate custom cells were simulated with a combination of ion channels from non-excitable cells and healthy human cardiomyocytes (hCMs). Using a genetic

SUBMITTER: Tieu A 

PROVIDER: S-EPMC9894098 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature