IsobaricQuant enables cross-platform quantification, visualization, and filtering of isobarically-labeled peptides.

ABSTRACT: In mass spectrometry (MS)-based quantitative proteomics, labeling with isobaric mass tags such as iTRAQ and TMT can substantially improve sample throughput and reduce peptide missing values. Nonetheless, the quantification of labeled peptides tends to suffer from reduced accuracy due to the co-isolation of co-eluting precursors of similar mass-to-charge. Acquisition approaches such as multistage MS3 or ion mobility separation address this problem, yet are difficult to audit and limited to expensive instrumentation. Here we introduce IsobaricQuant, an open-source software tool for quantification, visualization, and filtering of peptides labeled with isobaric mass tags, with specific focus on precursor interference. IsobaricQuant is compatible with MS2 and MS3 acquisition strategies, has a v

SUBMITTER: Hogrebe A 

PROVIDER: S-EPMC9894126 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature