Glutamine synthetase expression rescues human dendritic cell survival in a glutamine-deprived environment.

ABSTRACT:

Introduction

Glutamine deficiency is a well-known feature of the tumor environment. Here we analyzed the impact of glutamine deprivation on human myeloid cell survival and function.

Methods

Different types of myeloid cells were cultured in the absence or presence of glutamine and/or with L-methionine-S-sulfoximine (MSO), an irreversible glutamine synthetase (GS) inhibitor. GS expression was analyzed on mRNA and protein level. GS activity and the conversion of glutamate to glutamine by myeloid cells was followed by 13C tracing analyses.

Results

The absence of extracellular glutamine only slightly affected postmitotic human monocyte to dendritic cell (DC) differentiation, function and survival. Similar results were obtained for monocyte-derived macrophages. In contrast,

SUBMITTER: Schoeppe R 

PROVIDER: S-EPMC9894315 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature