A rapid and systematic approach for the optimization of radio thin-layer chromatography resolution.

ABSTRACT: Radiopharmaceutical analysis is limited by conventional methods. Radio-HPLC may be inaccurate for some compounds (e.g., ¹⁸F-radiopharmaceuticals) due to radionuclide sequester. Radio-TLC is simpler, faster, and detects all species but has limited resolution. Imaging-based readout of TLC plates (e.g., using Cerenkov luminescence imaging) can improve readout resolution, but the underlying chromatographic separation efficiency may be insufficient to resolve chemically similar species such as product and precursor-derived impurities. This study applies a systematic mobile phase optimization method, PRISMA, to improve radio-TLC resolution. The PRISMA method optimizes the mobile phase by selecting the correct solvent, optimizing solvent polarity, and optimizing composition. Without pr

SUBMITTER: Laferriere-Holloway TS 

PROVIDER: S-EPMC9894532 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature